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  • 名称:微波固定系统

  • 型号:Muromachi Microwave Fixation system TMW-4012C (10k
  • 厂商:其它品牌
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简单介绍:
微波固定系统,该设备能快速“固定”大脑化学物质,通过微波照射使大脑内的酶等化学物质维持不变,使得研究在活体条件下进行。系统能精确测量 cAMP, cGMP, GABA, Acetylcholine, choline, DOPA, 5HTP, endorphin, prostaglandins, and catecholines 以及它们的代谢物。 微波固定系统通过一个专门的发射器将产生的微波能量传
详情介绍:

微波固定系统

(实验动物脑内酵素瞬间灭活性系统)
Muromachi Microwave Fixation system


 原理与特色:

1. 用微波加热的方式使得神经组织内的酶(ENZYME)去活性,以避免死后变化
  (post-mortem changes)持续进展。
2. 此法比用冷冻固定法要来得好:冷冻法需要10-90秒做深入冷冻去活性,这段
    期间,深部组织的死后变化(post-mortem changes)已经在进行,因脑表面
    的热传导转差。微波法可在更短的时间内完全去活性。
3. 可以测:
               *  Acetylcholine乙酰胆碱
             
  * 
Choline 胆碱
               * 
Cyclic AMP 环腺苷酸
               * 
Cyclic GMP 环林鸟苷
               * 
GABA  伽马氨基丁酸              
               * 
DOPA 多巴(二羟基苯丙氨酸)
               * 
5-HTP 5-羟色胺酸
               * 
Serotonin 血清素
               * 
Endorphin 内啡肽
               * 
Prostaglandin 前列腺素
               *  
Catecholamines and their metabolites 儿茶酚胺及其代谢物
               *  Phosphorylated Proteins 磷酸化蛋白质

微波固化可以达到下列标准:

1. 可将温度升至75-90摄氏度,使脑部快速固定。
2. 微波能量可有效集中在动物的头部。
3. 各种动物间都可以达到相同的结果。
4. 对操作人员具备严格的保护,此设备相当简单安全


规格
振荡器 Toshiba Magnetron 2M68
微波输出 5kW max.
震荡频率 2.450±30MHz
冷却要求 水冷
水流量  每分钟两升
暴露时间 0.01-9.99秒 (0.01秒步进)
暂停时间 3 分钟
能量功耗 9.7千瓦  
频率 50 或者 60 Hz
电源要求 200/220VAC  40A  3Phase
外形尺寸 935W x 640D x 1,310H 厘米
重量 大约 220公斤

 






 

 

APPLICATOR HEAD

适用的动物管

TAW-174A WJM-24 for Mouse 15-20g
WJM-28 for Mouse 20-40g
TAW-424SA WJR-S for Rat 150-250g
TAW-424M WJR-M for Rat 250-400g
WJR-L for Rat 400-500g





水套式动物管(左侧的是大鼠固定器,右侧的是老鼠固定器)


标准系统包括:

Main Unit TMW-4012C 1
Applicator Head ( Any one from the list ) 1
Animal Holder ( Any one from the list ) 1
Dust Cover 1

发表文献:

Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 tesla

TB Rodrigues, IR Violante, S … - Magnetic Resonance in …, 2010 - interscience.wiley.com
... At the end of the infusions, cerebral metabolism was arrested using a 5- kW microwave
fixation system, the brain being rapidly removed from the skull and immediately frozen in liquid N2. ...

B Pardo, TB Rodrigues, L Contreras, M … - Journal of Cerebral …, 2010 - nature.com
... regulated circulating water bath. Fifteen minutes after 13 C-label administration, the
metabolism was arrested using a high-power (5 kW) focused microwave fixation system
The brain was ...

T Shinba, N Ozawa, M Yoshii, K Yamamoto - Neurochemical research, 2010 - Springer
... 4.5–5 kW, 1.4 s) using a small animal microwave applicator...
Immediately after microwave radiation, the brains were harvested and put onto an ice-chilled
Petri dish ... Moroji T, Takahashi T, Ogura K, Toishi T, Arai S (1977) Rapid fixation of rat ...

Altered Affect, Monoamine Transmitters and Bioenergetic Homeostasis of Alpha-synuclein-transgenic Mice, in the Presence and Absence of Endogenous Alpha- EM Cumyn - 2010 - tspace.library.utoronto.ca
... 36 3.7 Open field test..... 37 3.8 Microwave fixation and dissection of brain tissue.....
37 3.9 Fresh dissection and flash-freezing of brain tissue..... 40 ...

AY Taha, CT Chen, Z Liu, JH Kim, HTJ Mount, … - Neurochemical …, 2009 - Springer
... At 7 weeks of age, the mice were euthanized with a high-energy head-focused microwave
fixation system (6 kW, 0.88–0.99 s, Muromachi brain fixation system in order to stop brain lipid metabolism [14, 15]. ...

J Yang, C Johnson, J Shen - Journal of neuroscience methods, 2009 - Elsevier
... After in vivo MRS data acquisition of gabaculine-treated rats infused with [2,5- 13 C 2 ]glucose
and unlabeled glucose, the rats were euthanized using a microwave fixation device (Model
TNW-6402C, 5 kW, 1.5 s). Subsequently, the rat ...


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