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  • 名称:离体肺/肺切片灌注系统

  • 型号:ILSS
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ILSC 离体肺切片腔
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灌注系统概述

HSEHA生理学系统是一系列应用广泛的完备产品,可用于整体动物生理学、立体或在体器官和组织灌注及组织浴槽研究。这些系统具有广泛的应用,包括传统的安全药理学、DMPK、毒理学、呼吸系统、心脏病学、分子生物学和生理学。每个系统都设计了标准的组件,再加一些其他配置以适合特殊研究的需要。。标注组件也意味着如果你是需要将现有设备配齐,或者进行系统升级,或者是完整的配套产品,你都只需要购买需要的构件。HSE-HA生理学系统为你提供各种工具,使你的研究走向多产和成功。

我们提供大多数生理学检测设备,包括放大器、电极、传感器和电化学检测器。参数包括力、位移、压力、液体和气体流、心电图(单导联—12导联系统)、MAP(单个或多个部位)、脑电图、肌电图、连续支流灌注传统的血气参数和电解质的分析,可以选择程序化软件和传统的刺激器作为放大器、电极和传感器等产品的补充。

HSE-DAQ系统产品家族(BDASACADHAEMODYNISOHEARTPULMODYNPENNOCK)可以记录你的初始信号,计算到处的参数,注释数据,快速简易的输入确定的数据用于分析。同时还可以选择用于进一步分析的软件模块,带状记录纸打印,自动和控制模块等。产品经过预先设置,打开包装即可使用,并专门设计符合你的系统配置和数据分析要求。

 

 

• Assessment of lung functions under cell culture conditions

• From any species (murine, rat, human)

• Study of airways of different sizes

• Allows to quantify responsiveness of simple airways and simple vessels

• Analyzing ciliary beating frequency

Lung Functions under the Microscope

Precision-cut lung slices (PCLS) offer a novel and unique way to assess lung functions under cell culture conditions. They can be prepared from nearly any species including murine, rat and human lungs. The method allows the study of the response of airways of different size (down to the terminal bronchioles) and to relate these changes in lung functions to gene expression and mediator release. Slices are viable for at least three days. They can be placed under an inverted microscope, where digital image techniques allow to quantify not only the responsiveness of single airways, but also of single vessels. In addition, it is possible to analyze the ciliary beating frequency. More than 20 slices can be obtained from one lung, thus this

method is very economical in terms of experimental costs and animal use. Tissue cores are prepared from the lungs filled with agarose solution, after cooling to 4°C. From the cores, slices (220 ± 20µm) are cut using a Krumdieck tissue slicer

The incubation chamber was developed to allow incubation and observation of slices by an inverted microscope. The chamber is made of Polycarbonate. It is connected to a water bath to maintain constant temperature conditions. Two incubation cells are positioned in the center of the chamber. The bottom of the cells is sealed by glass, the cover is made of acrylic glass. The slices are fixed in the incubation cells by positioning them under nylon strings fixed to a bent platinum wire. The incubation cells can be filled with buffer, medium or drug solutions through the filling pipe. Buffer solution can be removed from the cells over a vacuum pipe. In addition, it is possible to gas the incubation cells in order to use bicarbonate buffered media.

The incubation chamber is placed on the stage of an inverted microscope and warmed to 37°C. The slices are screened for airways and transferred to the incubation chamber. Lung slices are selected for study using predefined criteria (Martin et al. 1996). Airways and vessels are focused, and finally the images are analyzed by image analysis software (e.g., Optimas or Metamorph).

 

Example of an Application

As an example Figure 1 shows exposure of a slice to increasing concentrations of endothelin-1. Shown is a lung slice containing an airway (B), a pulmonary artery (PA) and a pulmonary vein (PV). The pulmonary artery and the airway contracted almost completely, while the pulmonary vein area decreased to only 50% of its initial area. These responses are now easily quantified by digital imaging technique.

It is a distinct advantage of this technique that in many ways precisioncut slices can be treated like a cell culture. Thus, the slices can be incubated under various conditions and gene as well as protein expression or mediator release be determined. In contrast to cell culture models, in slices the anatomical structure of the lung is largely maintained, so that the functional consequences of gene expression and mediator release can be evaluated.

Catalog No. $ Product

BS4 73-2370Lung Slices Chamber

BS4 73-0125Thermostatic Circulator E 103, 230V AC, see page K27

Accessories

BS4 73-2802 Thermostatic Circulator E 103, 115V AC, see page K27

BS4 73-0113 Roller Pump Reglo Analogue ISM 827, 4 Channels, 0.002 to 30 ml/min

 

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