体外胚胎基因传递：如可进行小鼠体外胚胎基因传递（IVEGD）
体内基因传递：可用于体内，如将基因转入完整神经组织
BTX Genepaddles 是一种非插入式桨形可重复使用的电极。专为体内和体外应用，如鼠胚胎基因转移等而设计，适用于多个领域。这些电极头都是镀金的，有两种款式，每一种款式都包括了一对电极。在注射目的分子后，将电极置于胚胎前部和后部，使用BTX电穿孔系统施加电脉冲，Genepaddles可以用电极夹持器在组织内以一种预设的间隙平行放置。
Genepaddles有两种尺寸：3 x 5mm和5 x 7mm桨片。 可以适用于电穿孔靶区大小不同的需要。这些电极连接到45-0201方型柱式电缆，又通过45-0210香蕉形共轴电缆与脉冲发生器相接。
The BTX Genepaddles are paddle-style reusable electrodes suitable for a variety of applications. Genepaddles come in two models, each model consisting of a pair of electrodes. The Model 542 Genepaddles feature rectangular 3 x 5mm paddles, while the Model 543 Genepaddles feature rectangular 5 x 7mm paddles. The models vary to suit the size of the target electroporation area. These electrodes are connected to a Model 465 Square-Post Connection Cable and interfaced with a pulse generator via a Model 5343 Coaxial-banana Connection cable. The Genepaddles are positioned in parallel at a predetermined gap within a tissue using the Model 515 Genetrode Holder. Genepaddles may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Genepaddles have a life span of approximately 1500+ pulses, and are compatible with most BTX Electroporation Systems.
Applications
In Vitro Embryo Gene Delivery
Genepaddles were designed for In Vitro Embryo Gene Delivery (IVEGD) in mouse embryo. The electrodes are placed anterior and posterior to the embryo following injection of the molecule of interest, and an electroporation pulse is delivered using a BTX Electroporation System, such as the ECM® 830, 2001, 630, 600, and T820. The electric field introduced by the Genepaddles causes transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells. Itasaki1 used a 3 x 5mm Genepaddle-style electrode with a BTX T820 to deliver DNA to mouse embryos in vitro. His success demonstrates the potential of electroporation to transfect different types of tissues or embryos besides the classical avian embryo.
In Vivo Gene Delivery
Paddle-style electrodes have also been used for in vivo applications. Miyasaka2 used an electrode of this style with a BTX T820 to deliver DNA in vivo into the cerebral cortical anlage of E15 rat embryos. He demonstrated high-efficiency gene transfer into intact neural tissue using electroporation. Electroporation has the benefits of being less labor-intensive and more economical than competitive methods for in vivo gene transfer, such as viral methods and biolistics. Electroporation also produces greater viability and efficiency than competitive methods for in vivo gene transfer.
Technical Specifications
Standard Capabilities
Voltage Range: 0 - 200 V DC
Pulse Length Range: 1µsec - 99msec
Pulse Number Range: 1-99 (depending on voltage and pulse length settings)
Physical Characteristics
Model 542 Model 543
Paddle Dimensions: 3 x 5mm 5 x 7mm
Paddle Configuration: Rectangular Rectangular
Paddle Thickness: 1mm 1mm
Gold Plating Thickness: 0.04mm 0.04mm
All Genepaddle electrodes are gold plated, noninvasive electrodes
Generator Compatibility: ECM® 830, 2001, 630, 600, T820
Genetrode Holder Inter-electrode Gaps: 1-10mm
Ordering Information
Genepaddles Description Part Number
542 3 X 5 mm Genepaddles 10-002892-01
543 5 X 7 mm Genepaddles 10-002896-01
Accessories Description Part Number
515 Genetrode Holder 10-002560-01
465 Square-Post Cable 06-700044-01
5343 Banana-Coaxial Connection Cable 06-700042-01
4001 Enhancer 400 w/ Computer and Printer 01-001483-01
References
Itasaki et al., ''Shocking'' developments in chick embryology: electroporation and in ovo gene expression, Nature Cell Biology, 1: E203-E207 (1999) BTX Reference ID#4699
Miyasaka et al., Foreign gene expression in an organotypic culture of cortical anlage after in vivo electroporation, NeuroReport 10: 2319-2323 (1999) BTX Reference ID#464